Evaluation of Chemical, Ozone, and UV Methods in the Disinfection of Veterinary Operating Rooms at Veterinaria Guadalupe

Abstract

Introduction: Proper disinfection in veterinary operating rooms is essential to minimize the risk of infections in patients and staff. In Bolivia, specific disinfection protocols for veterinary clinics are limited and not legally regulated. This study addresses the implementation of a protocol that evaluates three disinfection methods: chemical (sodium hypochlorite), ozone, and UV radiation. Objective: To evaluate the disinfection protocol of the operating room at the Veterinary Clinic Guadalupe by comparing three methods to determine the most effective in reducing microorganisms. Methods: The study used a quantitative and descriptive approach, with initial and final environmental plating for each disinfection method. The chemical method, ozone, and UV radiation were compared and applied during alternating weeks. The results were analyzed using descriptive statistics. Results: Ozone showed the highest effectiveness in reducing Gram-positive microorganisms and fungi. UV radiation and chemical methods also reduced microorganisms but with lower overall effectiveness. Discussion-Conclusions: The implementation of ozone as a disinfection method in veterinary operating rooms is the most efficient, offering advantages in terms of effectiveness and environmental safety. The lack of specific protocols in Bolivian veterinary clinics highlights the need for adopting standardized evidence-based practices.